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Objective:To develop an HPLC method for determining matrine , oxymatrine and oxysophocarpine in wine-processed Sophora flavescens.Methods:An HPLC method was used with a Dalian Elite HYP-ODS column (250 mm ×4.6 mm, 5 μm).The mobile phase consisted of methanol-phosphate buffer (12:88) with the flow rate of 1.0 ml· min-1 .The detection wavelength was 205 nm, the column temperature was 25℃and the injection volume was 20 μl.Results:The calibration curve was linear within the range of 0.105-0.420 μg for matrine,0.760-3.040μg for oxysophocarpine and 1.615-6.460μg for oxymatrine.The average recovery of ma-trine, oxysophocarpine and oxymatrine was 99.70％(RSD=1.92％), 99.41％( RSD=1.93％) and 98.60％(RSD=1.79％)(n=6), respectively.Conclusion:The method is accurate and reliable with good reproducibility , promising specificity and high sensitivi-ty, which can be used for the quality control of wine-processed Sophora flavescens.
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Objective:To provide scentific evidence for determining the harvest period of Cortex Abizziae by studying the dynamic variation of the content of lignan glycoside I in Cortex Abizziae.Methods:Chromatography was performed on a Boston Green ODS C18 (250 mm×4.6 mm,5 μm) column,the mobile phase was acetonitrile-0.04% phosphate (18∶82),the flow rate was 1.0 ml·min-1,the detection wavelength was 204 nm,and the column temperature was at 25℃.Results:There were differences in the contents of lignan glycoside I in Cortex Abizziae at different harvest periods.The content of lignan glycoside I reached the highest level in January,decreased quickly from January to March,and gradually increased from April to December.Conclusion:The content of lignan glycoside I in Cortex Abizziae at different harvest periods is different,and the optimum harvest time of Cortex Abizziae is determined from November to January of the following year.
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Objective To establish the quality standard of Zhimiling Suppository (Cortex Phellodendri, Radix Sophorae Flavescentis, Catechu, Borneolum Syntheticum, Alumen). Methods Catechu, Borneolum Syntheticum, Alumen in the preparation were identified by physics and chemstry method. Berberine Hydrochloride was determined by HPLC. Results The standard curve for Berberine Hydrochloride was linear in the range of 0.080 2～0.802 ?g, the average recovery was 100.4%, RSD was 0.85% (n=6). Conclusion The methods are simple, accurate and reproducible.
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Objective To establish the method of the GC fingerprints for Ligusticum chuanxiong essential oil and its quality control.Methods The temperature at both feed inlet and detector was 250℃,the carrier gas was nitrogen,and the flow rate was 2 mL/min.GC of the essential oil in ten batches of L.chuanxiong was analyzed by adopting temprature programmer and the fingerprint peaks were identified by GC-MS.Results To set up the GC fingerprints for L.chuanxiong essential oil,13 peaks were marked out altogether and the GC fingerprints were analyzed by similarity analysis.Conclusion The method is of high degree of presion,reproducibility,and stability.The resolving of each component in the essential oil is good.The established fingerprints could be used as one of the quality control index for L.chuanxiong essential oil.
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AIM: To Establish the standard of Qirong Medicinal Wine (Radix Angelicae Sinensis, Fructus Psoraleae, Radix Polygoni Multiflori Preparata, Fructus Lycii and Flos Carthami, etc.). METHODS: Radix Angelicae Sinensis, Fructus Psoraleae, Radix Polygoni Multiflori Preparata, Fructus Lycii and Flos Carthami in the preparation were identified by TLC. Psoralen and isopsoralen were determined by HPLC. RESULTS: The average recoveries of psoralen and isopsoralen were 98.7% and 100.8%, respectively., RSD were 2.18%( n =5) and 1.67%( n =5),respectively. CONCLUSION: The methods are simple, accurate and reproducible.